MOTS-c (Mitochondrial Open Reading Frame of the 12S rRNA-c) is a 16-amino-acid mitochondrial-derived research peptide encoded within the mitochondrial genome rather than the nuclear genome. This makes it a notably distinct subject in metabolic and longevity research, as it is one of a small set of peptides synthesised from mitochondrial DNA. The Certificate of Analysis on this page documents in-house HPLC purity of the MOTS-c peptide at 99.64% in the production batch.
In-House Testing
BodyPharm laboratory
- Report
- #251210
- Batch
- MC328891
- Purity (HPLC)
- 99.64%
- Concentration
- 11.4 mg/ml
Testing Methodology
In-House HPLC Purity Verification
BodyPharm in-house report #251210 documents Reverse-Phase HPLC analysis of batch MC328891 at 99.64% purity, with concentration of 11.4 mg/ml in the pen formulation. MOTS-c is a 16-residue peptide that resolves as a single, well-defined peak on a standard C18 column under standard RP-HPLC conditions. Per-batch HPLC testing is repeated for every production run with a unique report number, and concentration is verified by integration against an internal reference standard at the time of fill.
Mitochondrial Peptide Stability
MOTS-c presents a stability profile distinct from nuclear-encoded peptides because of its hydrophobic character and tendency to associate with phospholipid membranes in research models. The pen formulation is buffered to maintain peptide stability at refrigerated temperatures (2-8C), and the HPLC test is performed shortly after fill. Researchers running extended-duration studies should reference the test date on their batch COA when planning study timelines, as MOTS-c stability in solution beyond the documented shelf life has not been characterised.
Synthesis Verification for a Mitochondrial-Encoded Sequence
MOTS-c is synthesised as a research compound by solid-phase peptide synthesis matching the 16-amino-acid sequence encoded by the 12S rRNA region of mitochondrial DNA. The HPLC retention time on the certificate is matched against a MOTS-c reference standard to confirm the synthesised material corresponds to the documented sequence. Mass-based identity verification is performed on representative batches and is available on request for institutional research procurement.
What This Result Means for Researchers
MOTS-c research has expanded since the peptide was first characterised in 2015 as a mitochondrial-derived signalling molecule. Research suggests MOTS-c functions as an exercise mimetic in metabolic research models, with documented effects on AMPK pathway activation, glucose homeostasis, and skeletal muscle metabolic flexibility. The peptide is investigated in models of metabolic disease, age-related metabolic decline, and mitochondrial dysfunction. Plasma MOTS-c levels have been reported to decline with age and to increase with exercise in human studies, making it a candidate biomarker as well as a research tool. The pen formulation supplies MOTS-c in research-grade purity for studies examining mitochondrial-nuclear communication and metabolic adaptation.
Frequently Asked Questions
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MOTS-C 32 Pen
MOTS-c 32mg pre-filled research pen with HPLC-verified purity at 99.64%, mitochondrial-derived peptide.